Results To observe single-cell dynamics of both healthy and epileptic networks, we used multibeam two-photon calcium imaging of the DG in hippocampal slices obtained from either an age-matched control group (n = 19 slices, 11 mice) or an epileptic group (n = 24 slices, 9 mice) in which mice had been subjected to the pilocarpine model of TLE (SI Materials and Methods).
Further electrophysiology and calcium imaging analyses demonstrated the maturation, connectivity and network properties of these cells in the astrocyte co-culture system (Fig. 5B). The isolated NPC population was also successfully transplanted into the developing DG of P10 animals to produce electrophysiologically active Prox1 + neurons.
In addition to a possible impairment in granule fusion events, several other factors may contribute to the reduction in insulin secretion present in ABCA1-deficient β-cells. Calcium imaging revealed a small increase in basal [Ca 2+] i levels and a decrease in the 2+]
Chromaffin cells only express two of the 17 known Syt isoforms: Syt-1 and Syt-7. Previous studies had identified that Syt-1 and Syt-7 possess distinct affinities for Ca2+ in the presence of phospholipid meranes and, once bound release meranes differentially.
data from granule cells loaded with the ratiometric Ca R+ sensitive dye fura-2. Measurements of [Ca 2+ ]i obtained with a duel wavelength fluorescence imaging system and IonVision software (Image processing and Vision Com- pany Ltd in this report and will
Urban monitored the subtle-yet-coordinated activity of populations of granule cells in living brain slices using calcium imaging, an optical imaging technique that has never been applied to
2017/11/14· Alzheimer’s disease (AD) is characterized by amyloid deposition, tangle formation as well as synapse loss. Synaptic abnormalities occur early in the pathogenesis of AD. Identifying early synaptic abnormalities and their underlying mechanisms is likely important for the prevention and treatment of AD. We performed in vivo two-photon calcium imaging to examine the activities of somas
2017/3/20· One method, called two-photon calcium imaging, had the resolution Wagner needed to study mouse granule cells in action. In order to study motor control, the team had to …
92 Cells CM-02, 1989 (unstained, X160) Identifiion Referee % Participant % Erythrocyte94.7 93.4 Fat – free fat droplets 5.3 2.3 Red blood cell cast - 2.0 This urine sample was obtained from a 34-year-old parturient patient who subsequently delivered a nor
2019/12/19· The Golgi cells, together with granule cells and mossy fibers, form a neuronal microcircuit regulating information transfer at the cerebellum input stage. Despite theoretical predictions, little was known about long-term synaptic plasticity at Golgi cell synapses. Here we have used whole-cell patch-clamp recordings and calcium imaging to investigate long-term synaptic plasticity at excitatory
classes of adult-born neurons (periglomerular neurons in the bulb [Livneh et al., 2014] and granule cells in the DG [Danielson et al., 2016]) using targeted patch-clamp recordings and GCaMP imaging, respectively. These studies also showed that immature adult-born
Cells were plated at approximately 14,500 cells/well in a 384 well plate. Ionized calcium binding adaptor molecule 1 (IBA1) is a 147 amino acid macrophage- and microglial-specific calcium binding protein, widely used as a marker for microglia.
One method, called two-photon calcium imaging, had the resolution Wagner needed to study mouse granule cells in action. High-res MRI links cerebellum to bipolar disorder In order to study motor
In a series of complex mice experiments using a state-of-the-art brain imaging technology called "two-photon calcium imaging," the Stanford researchers found that specific granule cells within the
The role of voltage-dependent Ca channels (VDCC) in the merane permeation of two toxic metals, lead (Pb) and cadmium (Cd), was studied in mammalian cells. Both metals interact with Ca-binding sites, but, while Cd influx appears to occur mainly through the same pathways as Ca, Pb is also rapidly taken up by different passive transport systems. Furthermore, I compared the effect of Cd in two
Title Cerebellar Granule Cells: Dense, Rich and Evolving Representations Published in Current Biology, 27(11), R415 - R418. Cell Press. ISSN 0960-9822. Author Badura, Aleksandra, De Zeeuw, Chris I Publisher Netherlands Institute for Neuroscience (NIN) Date
isolated purkinje cells. FAQ. Medical Information Search Calbindins S100 Calcium Binding Protein G Calcium Channels, P-Type Nerve Tissue Proteins Excitatory Amino Acid Transporter 4 Harmaline Serine-Type D-Ala-D-Ala Carboxypeptidase Calcium Channels, Q-Type Calcium Receptors, Metabotropic Glutamate Receptors, Glutamate NAV1.6 Voltage-Gated Sodium Channel Calbindin 1 …
Calcium imaging dataset accompanying the paper: Farzaneh Najafi, Gamaleldin F Elsayed, Robin Cao, Eftychios Pnevmatikakis, Peter E Latham, John Cunningham, Anne K Churchland. “Excitatory and inhibitory subnetworks are equally selective during decision-making and emerge simultaneously during learning” bioRxiv (2018): 354340.
Exploration for calcium requirements in carbachol-induced pigment granule dispersion To test whether an elevation in intracellular Ca 2+ was required for carbachol-induced pigment granule dispersion, RPE cells were isolated, treated with forskolin (FSK) and then challenged to disperse in the presence or absence of the merane-permeant Ca 2+ chelator BAPTA-AM.
We could hardly resolve individual cells in the granule cell layer though, presumably due to enhanced light stering and laser beam distortion in the Purkinje cell layer. Because multiple cellular structures are labeled using bolus loading, we next aimed to isolate calcium signals in the various compartments.
2017/6/15· Ca2+ imaging of dentate gyrus granule neurons further revealed that cytosolic Ca2+ increases induced by tetanic stimulation are reduced in PS cDKO granule neurons in acute hippocampal slices, and that inhibition of mitochondrial Ca2+ release during high
Calcium imaging with the confocal laser scanning microscope and ratio imaging analyses were performed as described above. Tissue Preparation for Light Microscopy After harvest, the leaf pieces were fixed either in 2% glutaraldehyde in 50 mM phosphate buffer, pH 7.6, for 30 min or in 95% ethanol for several hours at room temperature.
Granule cells in the cerebellum account for some 80 percent of the neurons in the brain, numerically, but only 10% of the brain’s volume. In order to watch how a mouse cerebellum does motor
2017/3/20· One method, called two-photon calcium imaging, had the resolution Wagner needed to study mouse granule cells in action. In order to study motor control, the team had to …
Other major interests of the group are the mechanisms of the input integration in granule cells (#5 published in 2016, #6 2013). To directly measure the propagation of signals along the dendritic arbors of granule cells we employ dendritic recordings, optical stimulation and calcium imaging, and verifiion of the obtained data by multicompartmental modelling.
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